Chromatography Septa i.e. pre slit septa and non slit septa
This article contains information about Chromatography Septa are available in different types and colors, using different materials, and the chemists main concern is whether I should choose Pre slit septa, this article will brief about difference between Pre slit septa and Non slit septa, This article is for reference only, the specific choice of that type of Septa need to be self-judgment.
Detailed information on pre slit septa and non slit septa
What is Pre Slit Septa ?
Pre-slit septa is ideally used for automatic sampling in HPLC, because Septa is kept sealed between Vials and Caps. Pre-slit processing makes it easy to puncture the sample feeder’s injection into HPLC Vials for sampling. Despite the slit, Septa is also able to maintain the sample seal in HPLC Vial without creating a vacuum in the air pressure inside the HPLC Vial when sampling the auto-injection needle.
Advantages of pre slit septa.
One of the main advantages of pre slit septa is that they can reduce the risk of coring, which is the formation of a small piece of septa material that can dislodge and contaminate the sample. When the septa are punctured by the needle repeatedly in the same spot, the septa material can distort and create a tiny core. Pre-slit septa reduce the chance of coring because the needle enters the septa through the pre-cut slit.
Pre slit Septa
Types of pre slit septa :
Two types of pre-slit Septa majorly used in laboratory, single slit and cross slit, such a slit can guide the injection needle accurately punctured Septa into the HPLC Vials, without a thin automatic injection broken, or the cushion falls off into the HPLC Vial contaminated sample.
Non slit septa
Non-slit septa help reduce carryovers from vials to vials because the re-sealing properties of the septum act as a skige for wiping the solution from the outside of the needle.
Typically, a non-slit septum exhibits resealing capabilities for a limited number of needle punctures , but it is recommended to use a new non-puncture septum when storing for long periods of time in vials.
How to Choose Pre slit septa and Non slit septa
There are a number of things to take into account when deciding between pre slit and non pre slit septa, including the sample type, needle gauge, and injection frequency.
Sample Type:
Pre slit septa may be necessary for some samples to lessen the chance of coring, but not necessarily requirement for all samples.
Needle Gauge:
A bigger needle gauge can need more force to get through non-pre-slit septa, which could cause damage to the septa or sample contamination. A pre-slit septum might be the best choice in this situation.
Injection Frequency:
A pre-slit septa may be more appropriate for applications requiring repeated injections since it can lessen the effort needed to penetrate the septa with a needle and increase the septa’s lifespan.
Frequently Asked Questions of Pre slit Septa.
Are Pre Slit Septa Reusable?
Primary Pre slit septa are generally intended for one use only and any attempt to reuse one could compromise the integrity of its seal, increasing contamination risks and decreasing accuracy and reliability results. Therefore it is advisable to use new septa for each sampling or analysis process for accurate and reliable results.
Are pre slit septa autoclavable?
The autoclavability of pre slit septa depends on their material composition; some septa may withstand autoclaving temperatures while others may degrade over time and lose sealing properties, so it is wise to consult your septa manufacturer’s specifications for autoclavability before using any autoclaved product. If autoclaving is required, alternative septa materials or sterilization methods may also be available.
Can pre slit septa be used with volatile or reactive samples?
Pre slit septa come in various materials that are designed specifically to be compatible with volatile or reactive samples, making it important to choose one suitable for such samples based on properties like high volatility or chemical reactivity. It’s best to seek manufacturer recommendations or consult a supplier when making this selection; otherwise, it could prove disastrous!
How should I store pre slit septa?
Pre-slit septa should be stored in clean, dry, and cool temperatures to preserve their integrity and preserve sealing properties. Exposure to high heat or humidity levels or direct sunlight could degrade them over time; for added protection, it would be prudent to store them in their original packaging or airtight containers to safeguard them from contamination while maintaining sealing properties.
Can pre slit septa be used with different needle sizes?
Pre slit septa are designed to accommodate various needle sizes. It is important that when selecting pre-slit septa for use with particular needle sizes the appropriate slit size matches up. This ensures a secure seal against leakage or damage of the septa. To select the right septa for your needle diameter use, consult either the manufacturer’s specifications or consult a supplier in order to select an ideal septa solution.
6.What Precautions to be taken while using Septa;
1. It is important to note that Silicone rubber can easily absorb chemicals from the environment.
2. Be careful when storing caps before use.
3. Do not store septums where chemical vapors are present for long periods of time.
4. I hope this article will be helpful for you to buy Septa.
Conclusion:
Necessity of your application will determine whether you should choose pre slit septa or non slit septa. While non-slit septa are advisable for applications requiring the highest level of sample integrity. pre-slit septa are more practical for normal use. You can make an informed choice and guarantee the accuracy and dependability of your chromatography analysis by being aware of the benefits and drawbacks of each type of septa.
