Simple Tips for HPLC Usages
Simple tips for HPLC usage
1. Always use high-quality solvents and reagents.
2. Use the correct hashtagcolumn for your sample type.
3. Keep the column clean and free of contaminants.
4. Use a hashtagguard column to protect the analytical column.
5. Use the correct mobile hashtagphase composition for your separation.
6. Optimize your hashtaggradient program for best results.
7. Keep the flow rate consistent throughout your run.
8. Use a hashtagdegasser to remove air bubbles from your mobile phase.
9. Check for hashtagleaks before starting your run.
10. Use a UV detector to monitor your eluent.
11. Calibrate your detector regularly.
12. Keep the detector cell clean and free of contaminants.
13. Use a hashtagreference wavelength to improve sensitivity and accuracy.
14. Avoid using buffers that can damage the column or detector.
15. Store columns properly when not in use.
16. Avoid exposing columns to extreme temperatures or humidity levels.
17. Flush the column with hashtagsolvent before starting a new run.
18. Monitor system pressure during runs to detect any issues early on.
19. Keep track of retention times for future reference and troubleshooting purposes.
20. Use a sample loop to inject precise volumes of sample onto the column.
21. Filter samples before injection to remove any particulates or impurities that could damage the column or detector
22.Use an autosampler for consistent and accurate injections
23.Use an isocratic method if possible, as it is simpler and more reproducible than gradient methods
24.Check pH of mobile phase, as it can affect separation efficiency
25.Avoid using too high of a flow rate, as it can cause band broadening
26.Use an appropriate injection volume based on sample concentration and sensitivity requirements
27.Check temperature settings, as temperature can affect retention times and separation efficiency
28.Use appropriate guard cartridges based on sample matrix complexity
29.Avoid overloading columns with too much sample, which can cause peak distortion and poor resolution
30.Check for column degradation regularly, as it can affect separation efficiency and peak shape
31.Use a column heater to improve separation efficiency and reproducibility
32.Use a column oven to maintain consistent temperature throughout the run
33.Avoid using too high of a mobile phase flow rate, as it can cause band broadening and poor resolution
34.Use a UV detector with appropriate wavelength settings for your analytes of interest
35.Check for baseline drift during runs, which can indicate detector or column issues
36.Avoid using too high of a sample concentration, which can cause peak distortion and poor resolution
37.Use an appropriate injection solvent based on sample solubility and compatibility with mobile phase
38.Check for air bubbles in the system before starting runs, which can cause pressure fluctuations and poor reproducibility
39.Avoid using dirty or contaminated syringes for injections, which can damage the column or detector
