Specification for Clear Mould glass vials (20 ml)

Description of Clear Mould glass vials (20 ml) :

Clear glass vials no foreign matter inside glass vials.

Physical Dimensions of Clear Mould glass vials (20 ml) :

Instrument: vernier caliper.

Procedure: Measure the at least 5 Nos. of 20 ml glass vials by vernier caliper and take

a Average.

     Instrument: vernier caliper

     Procedure: Measure the at least 5 Nos. of 20 ml glass vials by vernier caliper and take                              a   Average.

     Instrument: vernier caliper

     Procedure: Measure the at least 5 Nos. of 20 ml glass vials by micrometer and take a

Average.

     Instrument: Analytical Balance.

Weigh the 5 Nos. of 20 ml glass vials .Take a Average.

     Equipment: Measuring cylinder.

Fill the glass vials with distilled water & determine the overflow capacity .

Chemical Test of Clear Mould glass vials (20 ml):

Instrument: Water-Bath.

Heat the glass vials on water-bath  or in an air oven at about 50 °. Fill the vials with freshly prepared distilled water to the maximum volume compatible with sealing them by fusion of the glass & seal them.

Fill the vials 90 % of their calculated overflow capacity cover with aluminum foil previously rinsed with distilled water  .Place the  container in an autoclave ,displace the air by passage of steam for 10 minutes ,raise the temperature from 100 °C to 121°C over 20 minutes, maintain  a temperature of 121°C for 60 minutes & reduce the temperature from 121 °C to 100 ° C over 40 minutes, venting to prevent vacuum. Remove the vials from the autoclave & cool then  Combine the liquids from the vials.

Take 50 ml of volume of liquid for titration in conical flask. Add 0.15 ml of methyl red solution for each 50 ml liquid  .Titrate with 0.01 M hydrochloric acid taking as the end-point the colour obtained by repeating the operation using the same volume of freshly distilled water.

The difference between the preparations represents the volume of 0.01 M hydrochloric acid required by the test solution.

Calculate the volume of 0.01 M hydrochloric acid required for each 100 ml of test solution, if necessary

Instrument: Water Bath.

Pipette 10 ml of the test solution from the combined vials into conical flask, add 10 ml of nitric acid & evaporate to dryness on a water-bath.

Dry the residue in an oven at 130°C for 30 minutes. Cool, add to the residue 10.0 ml of hydrazine-molybate reagent, swirl to dissolve & heat under reflux on water-bath for 20 minutes.

cool to room temperature. Determine the absorbance of the resulting solution the maximum at about 840 nm.

Blank: 10.0 ml of hydrazine-molybate reagent 10.0 ml of hydrazine-molybate reagent.

Standard solution: Prepare the standard in the same manner of the test solution instead of the test solution using  0.1 ml of arsenic standard solution 10 ppm.