SOP for Bio Safety Cabinet

This Document describes (Standard Operating procedure) SOP for Operation, Maintenance and Cleaning of Bio Safety Cabinet.

SOP for Operation, Maintenance and Cleaning of Bio Safety Cabinet


  • To lay down the procedure for operation, maintenance and cleaning of bio safety cabinet.


  • This procedure is applicable for operation, maintenance and cleaning of bio safety cabinet


  • Microbiologist, officer and executive shall be responsible for the operation; maintenance and cleaning of bio safety cabinet procedure follow as per SOP.
  • Section head shall be responsible for the checking & compliance of the procedure as per SOP.


About the Bio safety cabinet

  • The bio safety cabinet delivers the air in the unidirectional flow in the vertical way which will flow downwards to the return grill of the cabinet.
  • The system is provided with prefilter, blower of suitable capacity, HEPA filters, magnehelic gauges and fluorescent lighting system with diffusers.
  • The system is also provided with UV [germicidal lamp]. The system also has UV hour meter for measuring the burning hours of the UV lamp and a UV timer to set the working period in   seconds/minutes or hours.
  • The system is provided with front sliding acrylic sash. The front sash is used to close the work area to prevent from the external contamination, open door only 150mm height if more than open    buzzer should be “ON” & red light blink.

Operation & maintenance procedure

  • Check the calibration / preventive maintenance status of the instrument before operation.
  • Connect the power cord of the bio safety cabinet to the main power supply and switch it “ON”.
  • Switch “ON” the cabinet blower and differential pressure of instrument shall be record as per respective annexure.
  • Disinfect the work surface, interior walls and surface of the window with 70% filtered IPA before and after use.
  • UV “ON” before 10 minutes carrying out any work to allow under the cabinet.
  • After 10 minutes switch “OFF” the UV light & switch “ON” the fluorescent light & carry out the activity to be done in under aseptic process.
  • Arrange the necessary microbiological testing aids in the cabinet without obstructing the air flow.
  • Perform procedures slowly by moving items in and out of the cabinet, avoid rapid movements.
  • Materials should be placed in the cabinet so as to not to block air flow.
  • Before starting the activity make sure that you have everything you need in the cabinet. The fewer times you pull your hands out of the cabinet, the less the disruption of the flow.
  • Switch “OFF” the airflow and switch “ON” the UV lamp for 10 minutes after completion of cleaning activity.
  • After 10 minutes switch “OFF” the UV lamp.
  • Bio Safety Cabinet switch “OFF” the lifter button to down side so that acrylic door of the bio safety cabinet shall move down side and close it.
  • Close the front acrylic door of the bio safety cabinet unit.
  • Record the detail of instrument operation and usages in Annexure “Instrument usage log book” (Annexure I).
  • Record the detail of UV lamp burning hour in Annexure “UV lamp burning record” (Annexure II).
  • If any abnormalities observed like differential pressure out of range of electrical problem then informed to engineering department through maintenance request for rectification and affix label on “Under maintenance” to the instrument.
  • Replace the UV lamp after reaching the manufactures recommended burning hours.
  • UV intensity shall be checked on monthly basis by LUX meter.


  • Before cleaning switch “OFF” the instrument and remove power cord from the main supply.
  • Initially clean the work bench with dry sterile mop wetted filtered 70% IPA.
  • Clean the entire instrument once in week with filtered 70% IPA or in case of any spillage or maintenance work.
  • Remove the prefilter from bio safety cabinet and clean on monthly basis it as per respective SOP.
  • Record the cleaning of bio safety cabinet as perAnnexure “Instrument cleaning record” (Annexure III).


  • UV lamp must be turned “OFF” when the room is occupied, to protect from direct exposure of UV light which can be burn the exposed part and cause the serious disease.
  • Incase if exposed & person is not feeling well, immediately seek for medical.
  • UV lamp must be cleaned with lint free cloth dampened with filtered 70% IPA to remove any dust& dirt that may block the germicidal effectiveness of UV lamp.
  • Do not spray 70% v/v IPA near the approximate of the flame of the LPG in the cabinet.


Sr. No. Annexure  No. Title of the Annexure
1. Annexure I Instrument usage log book.
2. Annexure II UV lamp burning hours.
3. Annexure III Instrument cleaning record.
4. Annexure IV Flow chart.


SOP Standard Operating Procedure No. Number
QA Quality Assurance A Annexure
QC Quality Control IPA Isopropyl Alcohol
% Percentage UV Ultra Violet
HEPA High Efficiency Particulate Air v/v Volume by Volume


    Annexure I

     Title: Instrument usage log book

Name of the Instrument Instrument ID No.
Sr. No. Date Blower Switch  ON Time Activity Start Time Activity Completion Time Blower Switch  OFF Time  

Used For

Performed By Checked By Remarks

Annexure II

Title: UV lamp burning hours

Instrument ID No. Instrument Location
Date UV Burning hour Total Time (Hrs) Total UV burning (Hrs) Done By Checked By Remark
Start Time End time



    Annexure III           

Title: Cleaning record of bio safety cabinet

Name of the Instrument Instrument ID. No.
Location Make/Model
Sr. No. Date of Cleaning Time Cleaning Agent Cleaned By Checked By Remark
Start End



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